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Monday, July 27, 2020 | History

2 edition of Immunofluorescent studies of microtubules in mouse peritoneal macrophages found in the catalog.

Immunofluorescent studies of microtubules in mouse peritoneal macrophages

Ronald E Ward

Immunofluorescent studies of microtubules in mouse peritoneal macrophages

by Ronald E Ward

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Published by Miami University in Oxford, Ohio .
Written in English

    Subjects:
  • Fluorescence microscopy,
  • Mice -- Anatomy

  • Edition Notes

    Statementby Ronald E. Ward
    The Physical Object
    Paginationiv, 41 leaves, typed :
    Number of Pages41
    ID Numbers
    Open LibraryOL14526962M

    In this study we evaluated changes in the secretion and activity of TNF-α from thioglycollate (TG)-elicited primary peritoneal macrophages and the mouse peritoneal macrophage cell lines RAW , PMJ-2R, and JA1 following exposure to LPS and hypoxia, where LPS represents gram-negative infection or ischemia-associated bacterial. In this study, we have examined the antitumor effects of an anti–mouse DR5 mAb, MD, against syngeneic tumor growth and metastasis in immunocompetent mice. This mAb not only inhibited the growth of TRAIL-sensitive tumor cells in vivo without toxicity, but also primed tumor-specific T cells that could eradicate TRAIL-resistant variants.

      Europe PMC is an archive of life sciences journal literature. The initial events during phagocytosis of latex beads by mouse peritoneal macrophages were visualized by high-resolution electron microscopy of platinum replicas of freeze-dried cells and by conventional thin-section electron microscopy of macrophages postfixed with 1% tannic acid. By exposing peritoneal macrophages to cytochalasin B, murine CoV infection changes from an acute cytopathology to a persistent type [52]. In SARS-CoV infection, these microtubule networks may.

    The principal approach was to study hemopoiesis on different stromal cell underlayers (fibroblasts or fibroblast-like cells covering a foreign body implanted into the peritoneal cavity of mice or. Taxol stabilizes microtubules in mouse fibroblast cells. Proc Natl Acad Sci USA 77 Crossref | PubMed | ISI Google Scholar; 39 Schmal H, Shanley TP, Jones ML, Friedl HP, Ward PA. Role for macrophage inflammatory protein-2 in lipopolysaccharide-induced lung injury in rats. J Immunol PubMed | ISI Google Scholar.


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Immunofluorescent studies of microtubules in mouse peritoneal macrophages by Ronald E Ward Download PDF EPUB FB2

Fig. Mouse 3T3 cell 10 hours after trypsinization. Microtubules appear to radiate from a central focus near the cell nucleus and extend outward into cell processes. 74 B. BRINKLEY ET AL. Mouse macrophages also display an extensive CMTC, which radiates from a single small region near the cell center (Frankel, ).Cited by:   This study shows the presence of acetylated tubulin in microtubules of mouse resi­dent peritoneal macrophages.

However, the comparative analysis using two distinct monoclonal antibodies designated as B and B-1 which specifically recognize all a-tubulins and only acetylated a-tubulin, respectively, reveals that there are relatively Cited by: 1.

Phorbol myristate acetate (PMA) stimulates cell spreading and fluid- phase pinocytosis in mouse peritoneal macrophages. Colchicine (10(-5) M) and cytochalasin B (10(-5) M) abolish PMA stimulated pinocytosis but have little effect on cellular spreading (Phaire-Washington et al.,J.

Cell Biol., ).Cited by: Trypanosome-activated mouse peritoneal macrophages phagocytized and digested Trypanosoma brucei in vitro and in vivo, but in the absence of specific antiserum and complement the degree of phagocytosis was minimal. Ultrastructurally, the parasites attached to the macrophage by their flagella, and ingestion proceeded flagellum by: By indirect immunofluorescence techniques, microtubules and mitochondria were localized in normal rat kidney cells, human WI38 fibroblasts, mouse peritoneal macrophages, and a putative smooth.

Cultured mouse peritoneal macrophages are highly susceptible to MHV-3 and the skeleton-disrupting drugs, and found that disruption of microtubules had no detectable effects on the infectious.

Patterns of cytoplasmic microtubules in somatic cell hybrids between transformed mouse cells and nontransformed human skin fibroblasts were examined using antitubulin antibodies as Immunofluorescent studies of microtubules in mouse peritoneal macrophages book immunofluorescent probe.

Nontransformed cells have been shown to exhibit an extensive cytoplasmic microtubule complex (CMTC), while in transformed cells, this complex is greatly diminished. To collect peritoneal macrophages, C57BL/6 wild type or apoE −/− mice (both are ~ 8 weeks old) were i.p.

injected with 3 ml 4% thioglycollate solution. About 5 days later, peritoneal macrophages were collected from mouse abdomen by lavage with PBS twice, and cultured in complete RPMI medium for 1 h followed by removal of the floating cells. Recent studies in this laboratory indicate that microtubules are important in the control of movement, spreading, and adhesion of mouse peritoneal macrophages (Cheung, et al., ).

Most of the experimental evidence for this model is based on the effect of colchicine on the migration, spreading, and adhesion of macrophages. Immunofluorescent staining showed that 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate-oxidized-low-density lipoprotein (Dil-ox-LDL)-positive cells were co-stained with F4/80, a marker of macrophages (Figure 1A–I), confirming the uptake of ox-LDL into mouse peritoneal macrophages.

By indirect immunofluorescence techniques, microtubules and mitochondria were localized in normal rat kidney cells, human WI38 fibroblasts, mouse peritoneal macrophages, and a putative smooth muscle rat cell line, in monolayer culture.

The mitochondria were found to be arranged along the cytoplasmic microtubules in each cell type. VIROL () Lactic Dehydrogenase Virus Replicates in Somatic Cell Hybrids of Mouse Peritoneal Macrophages and SVTransformed Human Fibroblasts SONDRA SCHLESINGER, ELIZABETH LAGWINSKA,2 CARLETON C.

STEWART,3 AND CARLO M. CROCE4 2 Department of Microbiology and Immunology, 'Cancer Biology, Washington University Medical. Isolation of mouse abdominal macrophages. Brewer thioglycollate (3 mL, Becton Dickinson) was injected into mice peritoneum 72 hours prior to cell harvest.

The peritoneal cavity was then injected with 5 mL PBS containing 3 mmol/L EDTA, and the fluid was collected and centrifuged to obtain macrophages. The oxidative metabolism of thioglycollate-elicited mouse peritoneal macrophages: the relationship between oxygen, superoxide and hydrogen peroxide and the effect of monolayer formation.

J Immunol. Sep; (3)– Tartakoff A, Vassalli P, Détraz M. Comparative studies of intracellular transport of secretory proteins.

Immunofluorescent staining. Cell lines and mouse peritoneal macrophages were stained with PE‐conjugated anti‐mouse CD47 (miap ) mAbs (BioLegend) or PE‐conjugated anti‐mouse CDa mAb (BD). Microscopic images were demonstrated at 40× magnification with a fluorescent microscope (BZ‐ Keyence, Osaka Japan).

Abstract. Mouse peritoneal macrophages were infected with varying numbers of Nocardia asteroidesand the fate of the ingested organisms was determined by viable plate count (VPC), light microscopy (LM), immunofluorescent microscopy (IM), and electron microscopy (EM).

The results obtained with these methods differed. VPC indicated that intracellular Nocardia decreased in numbers. fluorescein-labelled goat anti-mouse IgG preadsorbed on macrophage cultures (Mallucci, ). For acid phosphatase staining, an azo-dye-based method was used (Barka, ) and cells were.

Although fate-mapping studies have uncovered a great amount of detail on the origin and kinetics of fetal macrophage development in the yolk sac and.

tosis in mouse peritoneal macrophages. Colchicine ( M) and cytochalasin B ( M) abolish PMAstimulated pinocytosis but have little effectoncellular spreading (Phaire-Wash-ingtonetal., ,J.

CellBiol., ). Wereportherethat PMAalsoalters theorganization of thecytoskeleton and the distribution of organelles in these cells.

A study of indirect immunofluorescent and western blot analysis in 49 patients with scleroderma. Am J Clin Pathol. Oct;(4) Magro CM, Pope Harman A, Klinger D, Orosz C, Adams P, Waldman J, Knight D, Kelsey M, Ross P Jr.

Use of C4d as a diagnostic adjunct in lung allograft biopsies. PMA- or LPS-induced membrane ruffles are associated with C3bi-sRBCs. RAW mouse macrophages, primary mouse peritoneal macrophages and human monocyte-derived macrophages were isolated and plated as described in Materials and Methods.

Macrophages were serum-starved for 3 h before activation with nM PMA for 15 min or 10 μg/ml LPS for 6 h.(A–F) Neonatal mouse microglia (A and D), adult mouse microglia (B and E), or peritoneal mouse macrophages (C and F) were stained with mAb to mouse CD36 or isotype-matched control followed by FITC-labeled secondary antibody, visualized by fluorescence microscopy, and digitally photographed.Optimum insulin release occurred at a calcium concentration of mM.

Colchicine, in concentrations of 10() M, did not affect the microtubule immunofluorescent pattern, whereas concentrations of 1 and 5 x 10(-7) M decreased the number of microtubules, and microtubules could not be identified in cultures treated with 10(-6) M colchicine for 2 h.